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Refining metabolomic models: application of supervised methods and data filtering to different case-studies

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  • Date: - -
  • Venue: Collegio A. Volta, Pavia
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On December 13, 2019 Debora Paris, Consiglio Nazionale delle Ricerche (CNR) Istituto di chimica biomolecolare (ICB), will give a presentation entitled Refining metabolomic models: application of supervised methods and data filtering to different case-studies at 15:00 in the College Lecture theatre at Collegio A. Volta. All students are invited to attend, especially those reading Medicine, Biology, Biotechnology and Pharmaceutical Sciences.

The poster of the lecture can be downloaded here.

The most popular projection methods (PCA, PLS-DA and OPLS-DA) and their corresponding algorithms are nowdays implemented in many software and on-line resources,  so to be easily obtained and ‘automatically’ employed. Nevertheless, which one is the best to explore our data and to extract hidden relevant information? Although one can follow general rules, each case study can be considered apart, depending on the specific structure of data under investigation. In this context, different NMR-based metabolomics studies will be reported as examples of multivariate approach where different algorithms were applied in order to obtain biological information from noisy data in diverse applications. In particular, some statistical models will be briefly presented: the case of diatom Skeletonema marinoi collected in different growth phases; the in vivo application of the extremophile protozoo Fabrea salina exposed to UV radiation [1,2] and the analysis of metabolic profiles derived from exhaled breath condensate (EBC) collected from patients with stable and unstable cystic fibrosis [3].

[1] R. Marangoni, D. Paris, D. Melck, L. Fulgentini, G. Colombetti, A. Motta. Eur.Biophys.J.2012,41:107-114.
[2] R. Marangoni, D. Paris, D. Melck, L. Fulgentini, G. Colombetti, A. Motta. Biophys. J. 2011, 100:215-224.
[3] P. Montuschi, D. Paris, S. Montella, D. Melck, V. Mirra, G. Santini, N. Mores, E. Montemitro, F. Majo, V. Lucidi, A. Bush, A. Motta, F. Santamaria. American Journal of Respiratory and Critical Care Medicine. 2014, 190:229-233.

Currently a researcher at the CNR-ICB Institute of Biomolecular Chemistry of Pozzuoli, Napoli (Italy), Debora Paris has been working on NMR spectroscopy applied to metabolomics since 2004. She received the Graduation in Physics cum laude from the University of Napoli “Federico II” in the 2006 starting her experiences with the metabolomic study of the apoptotic process induced by ionizing radiation in MG-63 human spheroids by Nuclear Magnetic Resonance spectroscopy. She received the Ph.D in Chemistry from the University of Napoli “Federico II” in 2009, defending the PhD thesis “Development and Application of Advanced NMR Techniques for Biomarkers Identification”.

Main scientific interests:
-Research and development of specific NMR techniques for metabolomic investigations and two-dimensional experiments for fast acquisition (fast-NMR);
-Analysis and assignment of the metabolic profile of the spectral signals of various biological samples: microalgae, cells in vivo, cell extracts, tissue extracts, biofluids (blood, urine, saliva, respiratory condensate);
-Application of multivariate statistical data analysis, data projection and filtering methods and pattern recognition techniques for modeling, spectral classification and integration between data from different platforms.

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